Research Topics:



Other Protein studies from our lab

  • LOG Phosphoribonucleotide hydrolases
  • Thanks to a collaboration with Prof. I Frébort and P. Galuszka ( University of Holomuc, Czech Republic), we have been involved in structural characterization of a recently discovered class of proteins, named "Lonely Guy" or LOG. These are key enzymes of cytokinin biosynthesis, converting inactive cytokinin nucleotides into biologically active free-bases. We have determined the three-dimensional structure of a fungal LOG protein from C. purpurea in ligand-free and ligand-bound states. Our structural and biochemical analysis provided insights into the mechanism of substrate recognition in these enzymes and accurate sequence signature to identify Rossmann fold-containing LOG-like phosphoribonucleotide-processing enzymes, to distinguish them from mis- annotated lysine decarboxylases.

    Overview of the three-dimensional structure of cpLOG, rendered as cartoon. The reaction product phosphoribose, identified in the catalytic pocket of the enzyme, is shown as spheres Overview of the three-dimensional structure of cpLOG, rendered as cartoon. The reaction product phosphoribose, identified in the catalytic pocket of the enzyme, is shown as spheres.

    PDB Files:

    Raw Data in Repository:

    Publications: