Other Protein studies from our lab
Thanks to a collaboration with Prof. I Frébort and P. Galuszka ( University of Holomuc, Czech Republic), we have been involved in structural characterization of a recently discovered class of proteins, named "Lonely Guy" or LOG. These are key enzymes of cytokinin biosynthesis, converting inactive cytokinin nucleotides into biologically active free-bases. We have determined the three-dimensional structure of a fungal LOG protein from C. purpurea in ligand-free and ligand-bound states. Our structural and biochemical analysis provided insights into the mechanism of substrate recognition in these enzymes and accurate sequence signature to identify Rossmann fold-containing LOG-like phosphoribonucleotide-processing enzymes, to distinguish them from mis- annotated lysine decarboxylases.
|Overview of the three-dimensional structure of cpLOG, rendered as cartoon. The reaction product phosphoribose, identified in the catalytic pocket of the enzyme, is shown as spheres.|
Raw Data in Repository:
The three-dimensional Structure of Lonely Guy from Claviceps purpurea provides insights into the phosphoribohydrolase function of Rossmann fold-containing lysine decarboxylase-like proteins.
Proteins, 83, 1539-1546. (2015) *Shared Corresponding Authors - PubMed