LH1 ASP658ALA
SiMPLOD ID   This is the unique identifier for this mutation in the SiMPLOD database. Please use this identifier when linking information described in SiMPLOD about this mutation.	SiMPLOD1-82
Isoenzyme   Follow the links to gather information about the LH1 isoenzyme	Lysyl Hydroxylase 1 (human) - UniProt - Full Info
Mutation type   Current information about the clinical implications of the mutation	Mutation for Biochemical Studies (not necessarily related to observed polymorphisms)
Evidence at protein level   Based on available structural and biochemical information, a statement about the existence of an LH enzyme variant bearing the described mutation is provided	This variant EXISTS at the protein level: published experimental data support its existence as protein product.
LH Activity   When available, biochemical data describing the lysyl hydroxylase activity of the mutant are reported	-
References   Publications (and associated links) describing the mutation	Pirskanen et al., 1996 - DOI - PubMed
Notes from publications   A curated excerpt with information about the mutation from the publications found above	Pirskanen et al. performed site directed mutagenesis to characterize PLOD1 activity. Mutation of Asp658 completely inactivated lysyl hydroxylase activity. This is consistent with our PLOD3 structure where Asp669 (conserved in all PLOD) coordinates Fe2+
Structural Observations   An evaluation of the possible effects/implications of the mutations on the LH1 molecular structure	Coordinates Fe2+ in LH catalytic site
Related Entries   A list of related LH/PLOD variants found matching the structural position of the mutation currently visualized	SiMPLOD2-336: LH2a ASP668ALA (for biochemistry) SiMPLOD3-282: LH3 ASP669ALA (for biochemistry) SiMPLOD3-379: LH3 ASP669ASN (SNP) SiMPLOD3-380: LH3 ASP669HIS (SNP)
Last Update   An evaluation of the possible effects/implications of the mutations on the LH1 molecular structure	2021-06-23 08:38:51
The three-dimensional visualization is currently based on the homology model of full-length, dimeric human LH1 (generated using the crystal structure of full-length human LH3 as template). You may select a different PDB model file to visualize the mutation(s) using the drop-down menu below (page will refresh): LH1 homology modelLH3 with Fe2+ and 2-OG (PDB 6FXK)